Plasmid

Part:BBa_K4941037:Design

Designed by: Xinyu Chen   Group: iGEM23_ZJFH-Nanjing   (2023-10-08)


pylxp-CEN1-1-pTEF-Incomplete Intron-ylER-XPR2_terminator -ORI1001-Rep Origin (pMB1)-AmpR-Leu2 marker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 976
    Illegal XhoI site found at 1374
    Illegal XhoI site found at 5013
    Illegal XhoI site found at 5042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5899
    Illegal AgeI site found at 1853
    Illegal AgeI site found at 4428
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 822
    Illegal BsaI site found at 3607
    Illegal BsaI site found at 5184
    Illegal BsaI.rc site found at 237
    Illegal BsaI.rc site found at 5099
    Illegal BsaI.rc site found at 5382
    Illegal SapI site found at 1124
    Illegal SapI.rc site found at 4630


Design Notes

none


Source

yarrowia lipolytica

References